Syria, Iran and Russian Federation say Israel was behind an air strike on a Syrian air base on Monday that killed seven Iranian military personnel, something Israel has neither confirmed nor denied.
The news of the telephone call between Netanyahu and Putin appeared as Netanyahu took the stage in Israel's main Holocaust memorial event and issued a threat to Iran not to "test Israel's resolve".
Russian President Vladimir Putin called for refraining from actions destabilizing the situation in Syria during his phone conversation with Israeli Prime Minister Benjamin Netanyahu, the Kremlin press-service reported.
Israel is on high alert for any Iranian retaliation after Tehran's direct threat Tuesday, as well as any possible US strike against Syria's Assad regime in retaliation to the chemical attack at Douma.
The attack left 14 people dead, including seven Iranian soldiers. All three countries have blamed Monday's air strike on Israel.
Israel has struck Syrian positions dozens of times since the start of the war six years ago. Tel Aviv views Hizballah, an Iranian-backed regime ally, as its biggest threat and has grown alarmed over Tehran's growing clout in Syria.
The T-4 base had already been targeted by Israeli air raids in February, in retaliation for a drone sent to its territory by Iran.
Russian Federation on Wednesday also threatened to shoot down any possible USA missiles fired into Syria, as worldwide powers considered a unified response to a deadly chemical attack in the war-torn country, which the White House said Syria and Russian Federation were responsible for.