Products including locally popular polony sausages, also known as baloney, made at the Enterprise plant would be subject to immediate safety recalls, he added.
"The National Consumer Commission has issued the manufacturer Enterprise involved with food recall notices‚" Motsoaledi said.
Health Minister Aaron Motsoaledi said authorities in charge‚ which most likely fall under local government‚ would need to shut the Enterprise Polokwane factory down.
"Customers who bought any Enterprise product (including Bokkie‚ Renown‚ Lifestyle‚ Mieliekip) or any Rainbow ready-to-eat products‚ such as polony or Russians‚ can return the product for a full refund".
The foodborne disease, which is caused by bacteria found in soil, water, vegetation and animal faeces, has claimed over 170 lives, with over 900 cases reported since January 2017.
Motsoaledi said South Africa "can now conclude scientifically that the source of the present outbreak is the Enterprise Food production facility located in Polokwane".
"We advise members of the public to avoid all processed meat products that are sold as ready-to-eat", Motsoaledi said.
Its own tests had found "low detection of a strain of listeria in some products" Tiger Brands said. but had not yet confirmed the ST6 strain. "Also, over 10% of environmental samples collected by the EHPs at this facility have tested positive for L. monocytogenes", he said.
"While we know that polony is definitely implicated, there is a risk of cross-contamination of other ready-to-eat processed meat products, either at production, distribution or retail". These are an Enterprise facility in Germiston on the East Rand, and a Rainbow chicken facility in the Free State.
The polony from Rainbow Chicken has also been recalled.
National Consumer Commission deputy commissioner Thenzi Mabuza said "companies would have to come up with a plan on how they would recall all products which they must present on Monday morning".
"All fridges and food preparation areas in our stores will now be re-cleaned to prevent any cross-contamination".
"As an additional precautionary measure, we are recalling a limited number of viennas and cold meats".
Most of the current cases have been in Gauteng province, which includes the cities of Johannesburg and Pretoria, with other cases concentrated in Western Cape and KwaZulu-Natal provinces.
On Sunday the ministry of health announced a breakthrough in the listeriosis outbreak following several months of rigorous research. A total of 659 patients involve the outbreak strain, and 180 of those died, constituting a 27 percent fatality rate. The department also warned against other ready-to-eat meats which might be contaminated in various stages during manufacturing. Motsoaledi said it was at that stage of the investigation that officials chose to "visit all food-processing sites, food-packaging sites as well as food production sites where possible".
Tiger Brands has upgraded testing for listeria and introduced additional hygiene monitoring since the confirmed outbreak in December 2017, the company said.
While Listeria can not be spread from one person to another, pregnant women and infants, as well as people with weakened immune systems, are at a higher risk of contracting it.