Didi is the dominant ride-hailing company in China following its acquisition of Uber's Chinese business interests - a deal that still awaits full approval from competition authorities.
The latest investment values Didi at over US$50 billion, according to Reuters, and equips it with the cash needed to aggressively pursue expansion opportunities overseas.
On Thursday, Bloomberg reported that Didi is set to turn its attention to the Taiwanese franchise market, while earlier this month Reuters said it plans to expand into Mexico during the course of next year.
The ride-hailing company has already raised more than $13bn, with stakeholders including ecommerce rivals Alibaba and Tencent and U.S. tech giant Apple. Didi said on Tuesday that it was looking to bring its ride-hailing service to Taiwan.
Didi however, did not disclose the names of the investors in the round.
The company, which has announced plans to expand internationally earlier this year, establishing an worldwide arm in February. Didi also holds investments in Taxify, which operates in Europe and Africa, Brazil-based 99, as well as US-based Uber rival Lyft.
Three of the five most valuable startups on the planet - Didi, smartphone maker Xiaomi and Meituan-Dianping - are now Chinese, according to data from CB Insights.
"So far Mubadala has invested US$4-5 billion with Softbank as part of the Vision Fund", a Mubadala spokesperson told The National.
Abu Dhabi's Mubadala Investment and Japanese telecoms firm Softbank participated in Didi's $5bn funding round in July, and were widely reported to be among the global investors involved in the latest financing.